Location: Lisbon, Portugal
Who should take this course?
This course is intended for individuals who already work with method development in liquid chromatography. Chromatographers both from industry and universities will benefit from the course.
The course covers all aspects of method development from start to finish:
- what to think of before you start developing a method
- how the properties of the analyte and matrix should influence your approach
- the effects of operating variables and the mechanisms behind
- properties of different types of columns and mobile phases
- how to choose a column
- how to design a suitable chromatographic method
The factors that limit the precision and the accuracy of an LC method will also be discussed. The validation of methods is covered from a scientific perspective.
We will also provide the opportunity to discuss your own method development problems.
- Introduction to the course
- General aspects of method development
- Reversed phase chromatography
- Other modes of liquid chromatography
- Selecting the chromatographic mode
- The UV detector
- Other detectors
- Selecting the detector
- Initial steps of method development
- Adjusting separation conditions
- Checking system performance:
.....* Band broadening
- Isocratic or gradient?
- Optimizing the method:
.....* Optimizing resolution
.....* Optimizing speed
.....* Optimizing sensitivity
- Effect of changing:
.....* Column and particle dimensions
.....* Column chemistry
- RPLC column chemistry
- Column characterization
- Selecting the column
- Characterizing the method
What will you gain?
- The course will give you more confidence in your method development work.
- You will learn how fundamental principles can be applied in your method development work.
- You will also learn what to avoid when you develop LC methods and how to avoid building weaknesses into the methods you develop.
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