LIQUID CHROMATOGRAPHY IS A SCIENCE, NOT AN ART.  For more than 15 years I have regularly given a course in Advanced Chromatography for people from industry and universities. The course has been very much appreciated and some universities have run it as a part of their PhD program, qualifying for 5 credit points. A new format for the course has now been developed, the content being kept more or less the same. With internet and other communication technologies,

it is possible to follow the course from wherever you are and at your own pace. In addition, you will have live contact with me via chat programs. In this way it will be possible for you to address your own questions and other issues regarding the course.

COURSE FORMAT 

The course consists of six different sections. Each section consists of a 2 – 2½ hour long educational video presentation. You will have internet access to the video 24/7 during four weeks. After having watched the video, you may contact me and we will then schedule a personal live chat where we discuss the content of this particular section of the course. This procedure will ensure that you have understood the content of the section properly and it will give you the possibility to discuss any questions and concerns that you may have. After that, you go on to the next section, etc. 

The course starts in the autumn 2010 and ends in the spring 2011. The number of participants is restricted to 15. Furthermore, to ensure that you have the right qualifications to follow the course you must fill in an application. You can do that by sending an e-mail to me (jan@academyofchromatography.com) where you describe your education, your experience from HPLC and in a few sentences tell me why you would like to follow the course. 

Course content, brief description:

- Short review of important chromatographic concepts 
- Thermodynamics for chromatographers 
- Thermodynamics of retention 
- Molecular interactions 
- Regular solution theory and solubility parameters 
- Solution properties of the mobile phase 
- Adsorption isotherms and surface excess 
- Mass balance equation of chromatography 
- Non-linear and linear chromatography 
- Indirect detection 
- System peaks 
- Displacement chromatography 
- Gradient chromatography 
- Retention mechanism in reversed-phase chromatography of neutral solutes 
- Charged surfaces and the Poisson-Boltzmann equation

- Adsorption isotherm of small organic ions onto reversed phase surfaces. 
- Retention mechanism in reversed-phase chromatography of ionic solutes 
- Ion pair chromatography 
- Retention mechanism of small ions in ion exchange chromatography 
- Retention mechanism of proteins in ion exchange chromatography 
 
- Physical structure of column packings 
- Hydrodynamics and the Reynolds number 
- Poiseuille flow 
- Electroosmotic flow 
- Flow in packed columns 
- Diffusion 
- Dispersion in tubes 
- The Golay equation for dispersion in capillary columns 

- Dispersion in packed columns 
- Band broadening equations (van Deemter, Knox and others) 
- Analysis of column performance (column impedance, kinetic plots etc) 
- Concluding remarks and examples of how to apply physical theory to chromatography 

COURSE FEE 

For participants from the university; 950 €. 
For participants from the industry; 1950 € 

If there are more than three participants from the same place, contact me for a discussion regarding a reduction of the course fee. 

The course material will be sent to you when the course fee has been paid.

Jan Ståhlberg  Jan is Associate Professor in Physical Chemistry at Uppsala University and Adjunct Professor in Analytical Chemistry at Uppsala University . His research has mainly dealt with retention theories for ionic species, both small ions and macromolecules. migration theory in  electrochromatography and gradient chromatography. "Liquid chromatography is a multidisciplinary field with many interesting physico-chemical aspects which also have strong practical implications.

I have always been interested in theoretical problems with practical importance. Today, with the development of computers and software, it is possible to illustrate theoretical issues in a pedagogic way without mathematics.."   Jan has been working as an analytical chemist in the industry for more than thirty years. For more than 25 years he worked at the QA&QC department at AstraZeneca at different managerial positions and as Senior Scientific Adviser.   "More than thirty years of experience of developing analytical laboratories in the industry have taught me that professionalism at all levels in an organisation, is important for its performance. A good understanding of what you are working with is vital for all who want to be productive and do a solid work. Furthermore, the work is much more fun when you really understand what you are doing. This insight is the motto for my work in The Academy of Chromatography."